SLC38A2 and glutamine signalling in cDC1s dictate anti-tumour immunity

Tumour cell culture supernatant was collected 48 h later. Generation of CRISPR–Cas9 knockout tumour cell linesMC38 or B16-OVA cells were transduced with lentivirus of pLenti-Cas9-GFP (86145, Addgene). After sorting of Ametrine+ cells, cells were expanded, and deletion of SLC38A2 was verified by immunoblot analysis. Tumour cell culture supernatant was collected from medium cultured with MC38 or B16F10 cells in RPMI 1640 medium supplemented with 0.6 mM glutamine. Tumour cells and CD45+ immune cells from different mouse tumour models were pooled for analysis in GSE121861.