Image Recognition
The reaction was performed as follows; 42 °C for 10 min, 25 °C for 10 min, 50 °C for 60 min, 94 °C for 5 min. The touchdown PCR reaction was performed as follows; 95 °C for 5 min, [94 °C for 30 sec, 62 °C for 30 sec, 72 °C for 60 sec > 5x], [94 °C for 30 sec, 60 C for 30 sec, 72 °C for 60 sec > 2x], [94 °C for 30 sec, 55 °C for 30 sec, 72 °C for 60 sec > 2x], [94 °C for 30 sec, 52 °C for 30 sec, 72 °C for 60 sec > 25x], [94 °C for 30 sec, 56 °C for 30 sec, 72 °C for 60 sec > 10x], 72 °C for 10 min. The touchdown PCR reaction was performed as follows; 95 °C for 5 min, [94 °C for 30 sec, 62 °C sec for 30 sec, 72 °C for 60 sec > 5x], [94 °C for 30 sec, 60 °C for 30 sec, 72 °C for 60 sec > 2x], [94 °C for 30 sec, 55 °C for 30 sec, 72 °C for 60 sec > 2x], [94 °C for 30 sec, 52 °C for 30 sec, 72 °C for 60 sec > 25x], 72 °C for 10 min. The PCR reaction was performed as follows; 95 °C for 5 min, [94 °C for 30 sec, 50 °C for 30 sec, 72 °C for 55 sec > 50x], 72 °C for 10 min. Immunogenic characterization of isolated NiV F monoclonal antibodiesA pseudovirus neutralization assay was used because NiV is classified as a BSL-4 pathogen.